[1]夏凯,邵惠聪,陈珊珊,等.不同碳源对耐热型解脂亚罗酵母生长的影响[J].浙江科技大学学报,2024,(01):13-19,28.[doi:10.3969/j.issn.1671-8798.2024.01.002 ]
　XIA Kai,SHAO Huicong,CHEN Shanshan,et al.Effect of different carbon sources on growth of thermoresistant Yarrowia lipolytica[J].,2024,(01):13-19,28.[doi:10.3969/j.issn.1671-8798.2024.01.002 ]
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不同碳源对耐热型解脂亚罗酵母生长的影响(/HTML)
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《浙江科技大学学报》[ISSN:1671-8798/CN:33-1431/Z]

卷:
期数:
2024年01期
页码:
13-19,28
栏目:
出版日期:
2024-02-29

文章信息/Info

Title:
Effect of different carbon sources on growth of thermoresistant Yarrowia lipolytica
文章编号:
1671-8798(2024)01-0013-07
作者:
夏凯1; 2; 3; 邵惠聪1; 陈珊珊1; 彭艳婷1; 黄俊1; 2; 3
(1.浙江科技大学 生物与化学工程学院,杭州 310023; 2.浙江省农产品化学与生物加工技术重点实验室,杭州 310023; 3.浙江省农业生物资源生化制造协同创新中心,杭州 310023)
Author(s):
XIA Kai1; 2; 3;  SHAO Huicong1;  CHEN Shanshan1;  PENG Yanting1;  HUANG Jun1; 2; 3
(1.School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, Zhejiang, China; 2.Zhejiang Provincial Key Laboratory for Chemical and Biological Processing Technology of Farm Produce, Hangzhou 310023, Zhejiang, China; 3.Zhejiang Provincial Collaborative Innovation Center of Agricultural Biological Resources Biochemical Manufacturing, Hangzhou 310023, Zhejiang, China)
关键词:
解脂亚罗酵母;  赤藓糖醇;  耐热;  发酵
分类号:
Q815
DOI:
10.3969/j.issn.1671-8798.2024.01.002
文献标志码:
A
摘要:
【目的】探索不同碳源对耐热型解脂亚罗酵母生长及产赤藓糖醇的影响,通过优化碳源组合提升耐热型解脂亚罗酵母的赤藓糖醇产量。【方法】分别以30、34、38 ℃条件下生长的解脂亚罗酵母CA20、解脂亚罗酵母HT34和解脂亚罗酵母HT38为研究对象,利用含有不同碳源的培养基进行培养。培养过程中每隔24 h取样,采用酶标仪检测菌液在600 nm处的光密度值(optical density, OD600)。培养液中赤藓糖醇和葡萄糖质量浓度采用高效液相色谱(HPLC)检测。【结果】菌株CA20能利用葡萄糖、果糖、半乳糖、阿拉伯糖、木糖、麦芽糖、蔗糖、纤维二糖、甘露醇、山梨醇、甘油、可溶性淀粉和大豆油脂等碳源,其中葡萄糖、果糖、甘露醇、山梨醇、甘油和大豆油脂为最优碳源; 菌株HT34对除葡萄糖和果糖以外碳源的利用能力下降,而菌株HT38几乎不能利用除葡萄糖和大豆油脂以外的碳源; 培养基中同时添加葡萄糖和另一种碳源对菌株CA20生长的影响较小,而同时添加葡萄糖和大豆油脂可明显促进菌株HT34和HT38的生长。此外,大豆油脂可显著提升菌株HT34和HT38的赤藓糖醇产量和得率,增加量最高可达50%左右。【结论】本研究结果为今后利用高温发酵生产赤藓糖醇提供了理论依据。

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备注/Memo

备注/Memo:
收稿日期:2023-05-09
基金项目:国家级大学生创新创业训练计划项目(202211057026); 浙江科技学院科研启动基金项目(F701103L11); 浙江科技学院研究生课程建设项目(2021yjskj07)
通信作者:黄 俊(1979— ),男,河南省信阳人,教授,博士,主要从事蛋白质分子设计和定向进化及合成生物学研究。E-mail:huangjun@zust.edu.cn。
更新日期/Last Update: 2024-02-29