[1]朱玉珍,吴紫薇,高贻宙,等.特应性皮炎生物标志物鉴定及其免疫浸润研究[J].浙江科技学院学报,2023,(06):475-483.[doi:10.3969/j.issn.1671-8798.2023.06.003 ]
　ZHU Yuzhen,WU Ziwei,GAO Yizhou,et al.Identification of biomarkers in atopic dermatitis and study of its immune infiltration[J].,2023,(06):475-483.[doi:10.3969/j.issn.1671-8798.2023.06.003 ]
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特应性皮炎生物标志物鉴定及其免疫浸润研究(/HTML)
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《浙江科技学院学报》[ISSN:1001-3733/CN:61-1062/R]

卷:
期数:
2023年06期
页码:
475-483
栏目:
出版日期:
2024-01-01

文章信息/Info

Title:
Identification of biomarkers in atopic dermatitis and study of its immune infiltration
文章编号:
1671-8798(2023)06-0475-09
作者:
朱玉珍1; 吴紫薇1; 高贻宙1; UlfMeyer-Hoffert2; 吴志宏1; 2
(1.浙江科技学院 生物与化学工程学院,杭州 310023; 2.德国基尔大学 医学院皮肤学研究所,德国 基尔 24105)
Author(s):
ZHU Yuzhen1;  WU Ziwei1;  GAO Yizhou1;  Ulf Meyer-Hoffert2;  WU Zhihong1; 2
(1.School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, Zhejiang, China; 2.Department of Dermatology, University Hospital Schleswig-Holstein, Campus Kiel, 24105 Kiel, Germany)
关键词:
特应性皮炎;  生物信息学;  机器学习;  免疫浸润;  生物标志物
分类号:
R758.2
DOI:
10.3969/j.issn.1671-8798.2023.06.003
文献标志码:
A
摘要:
【目的】探索与特应性皮炎(atopic dermatitis,AD)相关的生物标志物,并分析免疫细胞浸润在该疾病中的病理作用。【方法】首先从基因表达综合数据库(Gene Expression Omnibus,GEO)下载与AD相关的转录组测序数据,并利用RStudio软件对数据集进行差异表达分析; 其次通过最小绝对收缩和选择算子回归分析(least absolute shrinkage and selection operator,LASSO)、支持向量机递归特征消除特征选择(support vector machine-recursive feature elimination,SVM-RFE)和随机森林(random forest,RF)3种机器学习算法,综合筛查与AD相关的生物标志物; 再次构建受试者工作特征(receiver operating characteristic,ROC)曲线,评估潜在生物标志物的预测价值; 最后通过估计RNA转录本的相对亚群(cell-type identification by estimating relative subsets of RNA transcripts,CIBERSORT)进行细胞类型识别,评估特应性皮炎的免疫细胞浸润情况,并分析潜在生物标志物与免疫浸润细胞之间的相关性。【结果】确定了2个与AD相关的生物标志物,即基质金属肽酶12(matrix metalloproteinase 12,MMP12)和WNT抑制因子1(WNT inhibitory factor 1,WIF1),并且通过ROC曲线说明了这两个基因的诊断价值较高; AD的发病机制与静息肥大细胞、滤泡辅助性T细胞、记忆CD4+T细胞、初始CD4+T细胞、树突状细胞、活化自然杀伤细胞、记忆B细胞及巨噬细胞相关。【结论】本研究结果为特应性皮炎的靶向治疗提供了新思路。

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备注/Memo

备注/Memo:
收稿日期:2022-09-23
基金项目:国家自然科学基金项目(81972922)
通信作者:吴志宏(1966— ),男,浙江省温州人,研究员,博士,主要从事皮肤天然免疫研究。E-mail:zhihong.wu@zust.edu.cn。
更新日期/Last Update: 2023-12-31